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Invention

Professor Ulrich K. Laemmli at the University of Geneva has developed new assays to map chromosomal proteins (e.g. transcription factors). These techniques overcome several limitations of the classical ChIP (Chromatin immunoprecipitation) such as high background and lack of resolution.

The methods consist of tethering micrococcal nuclease (MN) in an inactive state in vivo by fusion to a gene of interest (ChEC: Chromatin endogenous cleavage). Alternatively, MN is linked indirectly in vitro via antibodies to a target (ChIC: Chromatin immunocleavage). The nuclease is subsequently activated by Ca²⁺  addition that results in local doublestranded DNA cleavage. Standard tools of molecular biology are used to map the cleavage sites with high resolution and sensitivity. These assays can also be adopted for genome wide analysis.

Applications

Research tool: Improved diagnostic assay kit for the mapping of proteins along chromatin.

Advantages

• High specificity
• Greater precision: up to bp resolution
• Increased signal to noise ratio
• Applicable to structural/insoluble proteins
• Applicable to both fixed and native cells

Contacts

Université de Genève
Dr. Alexandra Richardson
alexandra.richardson(at)unige.ch

Dr. Céline Lafourcade
celine.lafourcade(at)unige.ch

Additional information is provided in the attached pdf.

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