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DNA typing of distinct STR loci establishes the identification of individuals or biological samples collected at crime scenes since several years. If only low amounts of DNA are available and /or the quality of the DNA is rather poor due to degradation, with commercial multiplex kits the typing often results in no or only partial DNA profiles.

The innovation comprises a method in which two PCR reactions (a sixplex and a fiveplex PCR) are combined into one large multiplex reaction. After amplification the preparation is splitted and specifically labelled amplicons are further analysed.

Application

The innovative BioPlex-11 multiplex PCR system provides a significant and sensitive system for a powerful analysis of degraded and low copy DNA samples. It allows the simultaneous analysis of ten short amplicon STR systems from a single aliquot.

Advantages

• Samples were successfully typed down to 25 pg of DNA (equivalent of approx. four cell nuclei) demonstrating the high sensitivity of the assay
• Correct genotypes were obtained for highly degraded DNA samples
• The assay could be applied for analyses of telogen hair roots and for genotyping of tsunami cases

Stage of Development

• Specificity and reproducibility of the method was verified by testing 15 blood samples
• Application of the assay in practical work of analysing telogen hair roots from real casework  

Contact

IMG Innovations-Management GmbH
Dr. Wolfgang Stille 
stille(at)img-rlp.de

About IMG

The IMG Innovations-Management GmbH is a subsidiary of the Investment and Economic Development Bank of Rhineland-Palatinate GmbH. It offers a multitude of services for the realisation and commercialisation of innovations and inventions including intellectual property protection and license brokerage. It is the primary contact point regarding all matters of technology and knowledge transfer within the German state of Rhineland-Palatinate. Within the framework of the Scientific Patent Network Rhineland-Palatinate it forms the connection between scientists and university administrations and provides universities and research institutions with all prerequisites to bring research results to the market on a fast track. Within the Scientific Patent Network the IMG is the leading service provider offering as a proficient partner a professional intellectual property management to all associated universities and institutions.    

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